No, GloFish should not be housed with goldfish. GloFish are tropical fish requiring warmer water temperatures (72-82°F), while goldfish are coldwater fish preferring cooler temperatures (65-72°F). Their differing needs make them incompatible tank mates.
Water temperature is critical because each fish species has an optimal range for their metabolic functions, immune system, and overall health. Forcing glofish and goldfish to live in a compromised temperature range can lead to chronic stress, weakened immune systems, increased susceptibility to disease, and a significantly shortened lifespan for both. It directly impacts their well-being.